S100A4 Antibody (1C4) - BSA Free

Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 recombinant protein with S100A4 antibody (clone 1C4) at 0.5 ug/ml concentration.

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1C4) [NBP2-36431] - S100A4 (1C4) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration.

Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on THP-1 cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 in lysates of A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa.

Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophages) developed nuclear staining also.

Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Aanalysis of tissue section of Squamous Cell Carcinoma of Human Lungs with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. An expected cytoplasmic as well as nuclear staining was observed in the tissue and the staining was found to be more intense in several cells which are potentially the advanced cancer cells, lymphocytes or infiltrating macrophages.

Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human colon with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The columnar epithelial cells showed weak to negligible immunopositivity whereas the cells present in lamina propria, the loose cellular connective tissue in mucosa, developed intense staining in various type of cells.

Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human skeletal muscle with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The different myocytes showed a varying degree of cytoplasmic immuno-positivity whereas the staining was found to be intense in some of the peripherally placed nuclei.

Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on HeLa cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, KO
• Clone: 1C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

S100A4 Antibody (1C4) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse S100A4 Antibody (1C4) - BSA Free (NBP2-36431) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-S100A4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partial recombinant human S100A4 protein (between amino acids 1-200) [UniProt P26447].
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockout Validated
  • Western Blot 1.0 ug/ml
• Theoretical MW: 11.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for S100A4 Antibody (1C4) - BSA Free

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 have been implicated in tumor metastasis. Recombinant mouse S100A4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A4 Antibody (NBP2-36431)(2)

Publications using NBP2-36431

• Sulaiman R, De P, Aske JC et al. Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs International journal of molecular sciences 2023-03-29 [PMID: 37047422] (FLOW, Human)
• Chiarelli N, Zoppi N, Ritelli M et al. Biological insights in the pathogenesis of hypermobile Ehlers-Danlos syndrome from proteome profiling of patients' dermal myofibroblasts Biochimica et biophysica acta. Molecular basis of disease 2020-12-28 [PMID: 33383104] (WB, Human)

Bioinformatics

• Gene Symbol: S100A4
• Entrez:
  • 6275(Human)
• Uniprot:
  • P26447()