RWDD3 Antibody - BSA Free

Western Blot: RWDD3 Antibody [NBP1-76310] - Rat kidney tissue lysate with RWDD3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RWDD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RWDD3 Antibody - BSA Free (NBP1-76310) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3. The immunogen is located within the last 50 amino acids of RWDD3. Amino Acid Squence: KEYSALDELQKEFET
• Predicted Species: Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RWDD3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
• Control Peptide:

  RWDD3 Peptide (NBP1-76310PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RWDD3 Antibody - BSA Free

• DKFZp566K023
• FLJ51225
• RSUME
• RWD domain containing 3
• RWD domain-containing protein 3
• RWD-containing sumoylation enhancer

Background

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappabeta sumoylation and stability. In addition, RWDD3 inhibits TNF-alpha-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappabeta increased stability. RSUME-enhanced sumoylation of Ikappabeta leads to the inhibition of NF-kappabeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RWDD3
• Uniprot:
  • NP_056300(Human)