RUNX2/CBFA1 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

RUNX2/CBFA1 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal region of the human RUNX2 protein (within residues 225-300). [Swiss-Prot Q13950]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUNX2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This RUNX2 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RUNX2/CBFA1 Antibody [FITC]

  • Acute myeloid leukemia 3 protein
  • AML3CCD
  • CBFA1
  • CBFA1MGC120022
  • Core-binding factor subunit alpha-1
  • MGC120023
  • PEA2-alpha A
  • PEBP2A
  • PEBP2A1CCD1
  • PEBP2aA1
  • PEBP2-alpha A
  • runt domain, alpha subunit 1
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

RUNX2 is a transcription factor that mediates the expression several downstream proteins, which affect osteoblast differentiation and mitotic progression. It also is important in monocyte activation, as it is upregulated in response to LPS infection. Through interaction with Twist 1 and Twist 2, RUNX2 regulates osteocalcin transcription, following parathyroid hormone signaling for bone loss repair. Ossification in skeletal formation is also partially regulated through RUNX2. Essentially, this transcription factor is responsible for many developmental and repair mechanisms in bone and collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for RUNX2/CBFA1 Antibody (NBP1-77462F). (Showing 1 - 1 of 1 FAQ).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.

Secondary Antibodies

 

Isotype Controls

Additional RUNX2/CBFA1 Products

Bioinformatics Tool for RUNX2/CBFA1 Antibody (NBP1-77462F)

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Diseases for RUNX2/CBFA1 Antibody (NBP1-77462F)

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Pathways for RUNX2/CBFA1 Antibody (NBP1-77462F)

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PTMs for RUNX2/CBFA1 Antibody (NBP1-77462F)

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Bioinformatics

Gene Symbol RUNX2
Entrez