RUNX2/CBFA1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

RUNX2/CBFA1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
  Lys219-Tyr404
  Accession # Q13950
• Specificity: Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX2/CBFA1 Antibody [Alexa Fluor® 594]

• Acute myeloid leukemia 3 protein
• CBFA1
• CBF-alpha-1
• CCD1
• CCDAML3
• CLCD
• Core-binding factor subunit alpha-1
• core-binding factor, runt domain, alpha subunit 1
• MGC120023
• ML3
• oncogene AML-3
• OSF2
• OSF-2
• osteoblast-specific transcription factor 2
• PEA2aA
• PEA2-alpha A
• PEBP2A
• PEBP2aA
• PEBP2-alpha A
• polyomavirus enhancer-binding protein 2 alpha A subunit
• runt domain, alpha subunit 1
• runt related transcription factor 2
• runt-related transcription factor 2
• RUNX2
• SL3/AKV core-binding factor alpha A subunit
• SL3-3 enhancer factor 1 alpha A subunit

Background

Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for RUNX2/CBFA1 Antibody (AF2006T). (Showing 1 - 1 of 1 FAQ).

1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
   • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.