RUNX1/CBFA2 Antibody - BSA Free

Western Blot: RUNX1/CBFA2 Antibody [NB100-2044] - Used at 5 ug/ml to analyze AML1 in (A) human Ramosand (B) mouse Raw cell lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

RUNX1/CBFA2 Antibody - BSA Free Summary

• Immunogen: Rabbit anti-AML1 polyclonal antibody was raised against a peptide corresponding to a sequence that is homologous in AML1 and AML1/ETO (amino acids 231 to 245 of human origin).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RUNX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5 ug/ml
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  RUNX1/CBFA2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for RUNX1/CBFA2 Antibody - BSA Free

• Acute myeloid leukemia 1 protein
• AML1
• AML1acute myeloid leukemia 1
• AML1-EVI-1
• CBFA2
• CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1
• Core-binding factor subunit alpha-2
• EVI-1
• PEBP2A2
• runt domain, alpha subunit 2
• runt-related transcription factor 1
• RUNX1
• SL3/AKV core-binding factor alpha B subunit

Background

AML1 is a DNA-binding protein which specifically interacts with a sequence belonging to the group of enhancer core motifs, TGT/cGGT. The AML1 gene on chromosome 21 is disrupted in the (8;21)(q22;q22) translocation associated with acute myelogenous leukemia and encodes a protein with a central 118-amino-acid domain with 69% homology to the Drosophila pair-rule gene, runt. AML1 translocation is frequently observed in acute myeloid leukemia. It is a key transactivator of tissue-specific genes of the hematopoietic and bone lineages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNX1
• Entrez:
  • 861(Human)
  • 12394(Mouse)
• Uniprot:
  • Q01196()