RTN3 Antibody (PSH0-40)

Western Blot: RTN3 Antibody (PSH0-40) [NBP3-32921] - Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 ug/Lane) Lane 2: HEK-293 cell lysate (33 ug/Lane) Lane 3: U87-MG cell lysate (30 ug/Lane) Lane 4: A549 cell lysate (26 ug/Lane) Lane 5: SH-SY5Y cell lysate (18 ug/Lane) Lane 6: PC-12 cell lysate (23 ug/Lane) Lane 7: L6 cell lysate (14 ug/Lane) Lane 8: Human cerebellum tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 112.6 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32921) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: RTN3 Antibody (PSH0-40) [NBP3-32921] - Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: RTN3 Antibody (PSH0-40) [NBP3-32921] - Flow cytometric analysis of HeLa cells labeling RTN3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32921, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: PSH0-40
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RTN3 Antibody (PSH0-40) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human RTN3 aa 1-500 / 1,032. (Uniprot: O95197)
• Localization: Endoplasmic reticulum membrane, Golgi apparatus membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RTN3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 112.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RTN3 Antibody (PSH0-40)

• ASYIPASY interacting protein
• HAP
• isoforme III
• isoforme VI
• Neuroendocrine-specific protein-like 2
• Neuroendocrine-specific protein-like II
• NSPL2NSP-like protein II
• NSPLIIhomolog of ASY protein
• NSP-like protein 2
• reticulon 3
• reticulon-3
• RTN3-A1

Background

May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis. There are 5 isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RTN3