RSK1 [p Thr359] Antibody

Western Blot: RSK1 [p Thr359] Antibody [NBP3-13253] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Thr359) antibody (NBP3-13253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Po, Bv, Ch, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RSK1 [p Thr359] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr359 of human RSK1. The exact sequence is proprietary.
• Modification: p Thr359
• Predicted Species: Porcine (100%), Bovine (100%), Xenopus (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RSK1 [p Thr359] Antibody

• 90kD, polypeptide 1)
• EC 2.7.11
• EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
• HU-1
• MAP kinase-activated protein kinase 1a
• MAPK-activated protein kinase 1a
• MAPKAP kinase 1a
• MAPKAPK-1a
• MAPKAPL1A
• p90RSK1
• p90S6K
• ribosomal protein S6 kinase alpha 1
• ribosomal protein S6 kinase alpha-1
• ribosomal protein S6 kinase, 90kD, polypeptide 1
• ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Ribosomal S6 kinase 1
• RPS6KA1
• RSK
• RSK1
• RSK-1
• RSK1p90-RSK 1
• S6K-alpha 1
• S6K-alpha-1

Background

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA1