RSK1 [p Thr359, p Ser363] Antibody (RM233)

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (RM233) [NBP2-61584] - Western Blot of A431 cells treated (+) or nontreated (-) with EGF, using NBP2-61584, showed a band of phosphorylated Rsk1 only in EGF treated A431 cells .

Immunohistochemistry-Paraffin: RSK1 [p Thr359, p Ser363] Antibody (RM233) [NBP2-61584] - Immunohistochemical staining of formalin fixed and paraffin embedded human cerebral cortex tissue sections using NBP2-61584.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: RM233
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

RSK1 [p Thr359, p Ser363] Antibody (RM233) Summary

• Description: Novus Biologicals Rabbit RSK1 [p Thr359, p Ser363] Antibody (RM233) (NBP2-61584) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A phospho-peptide corresponding to Human RSK1 [p Thr359, p Ser363]
• Modification: p Thr359, p Ser363
• Specificity: This antibody reacts to human RSK1 only when phosphorylated at Thr359/Ser363.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS6KA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for RSK1 [p Thr359, p Ser363] Antibody (RM233)

• 90kD, polypeptide 1)
• EC 2.7.11
• EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
• HU-1
• MAP kinase-activated protein kinase 1a
• MAPK-activated protein kinase 1a
• MAPKAP kinase 1a
• MAPKAPK-1a
• MAPKAPL1A
• p90RSK1
• p90S6K
• ribosomal protein S6 kinase alpha 1
• ribosomal protein S6 kinase alpha-1
• ribosomal protein S6 kinase, 90kD, polypeptide 1
• ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Ribosomal S6 kinase 1
• RPS6KA1
• RSK
• RSK1
• RSK-1
• RSK1p90-RSK 1
• S6K-alpha 1
• S6K-alpha-1

Background

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA1