RPL22 Antibody - Azide and BSA Free

Western Blot: RPL22 Antibody [NBP2-93082] - Western blot analysis of extracts from normal (control) and RPL22 knockout (KO) 293T cells, using RPL22 antibody (NBP2-93082) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP2-93082] - Rat lung using RPL22 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western Blot: RPL22 Antibody [NBP2-93082] - Analysis of extracts of various cell lines, using RPL22 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RPL22 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1). MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL22
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for RPL22 Antibody - Azide and BSA Free

• EAPHBP15
• EBER-associated protein
• Epstein-Barr virus small RNA-associated protein
• Epstein-Barr-encoded RNA-associated protein
• HBP15/L22
• heparin-binding protein 15
• Heparin-binding protein HBp15
• ribosomal protein L22,60S ribosomal protein L22

Background

RPL22, also known as 60S ribosomal protein L22, is a 15kDa 128 amino acid protein. The primary function of RPL22 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL22 in relation to hepatitis, carcinoma, malaria, leukemia, hepatitis c, pneumonia and lung carcinoma. RPL22 has been linked to the virulence, methylation, contact inhibition, pathogenesis and cell growth pathways where it interacts with SHC1, UBA5, CSE1L, PRKAG1 and MAP3K14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: RPL22