RPL13 Antibody - BSA Free

Western Blot: RPL13 Antibody [NBP2-94875] - Analysis of extracts of various cell lines, using RPL13 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.

Immunocytochemistry/Immunofluorescence: RPL13 Antibody [NBP2-94875] - Analysis of MCF7 cells using RPL13 . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RPL13 Antibody [NBP2-94875] - Rat spleen using RPL13 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: RPL13 Antibody [NBP2-94875] - Human lung using RPL13 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: RPL13 Antibody [NBP2-94875] - Rat brain using RPL13 antibody at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPL13 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPL13 Antibody - BSA Free (NBP2-94875) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1). MAPSRNGMVLKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPIRPIVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPRRRNKSTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNVYKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL13
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RPL13 Antibody - BSA Free

• BBC1FLJ27454
• Breast basic conserved protein 1
• D16S444E
• FLJ27453
• MGC117342
• MGC71373,60S ribosomal protein L13
• OK/SW-cl.46
• ribosomal protein L13

Background

RPL13, also known as 60S ribosomal protein L13, is a 24kDa 211 amino acid protein with a shorter 19kDA 164 isoform produced by alternative splicing. RPL13 belongs to the L13E family of ribosomal proteins and can be found in the cytoplasm. Current research is being conducted on RPL13 in relation to Smith-Magenis syndrome, Treacher Collins syndrome, breast carcinoma, carcinoma, skin atrophy, prostate cancer, prostatitis, schizophrenia and malaria. RPL13 has been linked to the reverse transcription, ribosome assembly, cell division, DNA protection and pigmentation pathways where it interacts with RPL4, RPL8, RPS3, SMN1 and RPL12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPL13