RPC62 Antibody - Azide and BSA Free

Western Blot: RPC62 Antibody [H00010623-B03P] - Analysis of POLR3C expression in human placenta.

Immunocytochemistry/Immunofluorescence: RPC62 Antibody [H00010623-B03P] - Analysis of purified antibody to POLR3C on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: RPC62 Antibody [H00010623-B03P] - Analysis of POLR3C expression in Hela S3 NE.

Western Blot: RPC62 Antibody [H00010623-B03P] - Analysis of POLR3C expression in PC-12.

Western Blot: RPC62 Antibody [H00010623-B03P] - Analysis of POLR3C expression in transfected 293T cell line. Lane 1: POLR3C transfected lysate (58.74 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RPC62 Antibody - Azide and BSA Free Summary

• Immunogen: POLR3C (NP_006459.3, 1 a.a. - 534 a.a.) full-length human protein. MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
• Specificity: POLR3C - polymerase (RNA) III (DNA directed) polypeptide C (62kD),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: POLR3C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and transfected lysate for Western Blot. Has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RPC62 Antibody - Azide and BSA Free

• DNA-directed III 62 kDa polypeptide
• DNA-directed RNA polymerase III subunit C
• DNA-directed RNA polymerase III subunit RPC3
• polymerase (RNA) III (DNA directed) polypeptide C (62kD)
• RNA polymerase III 62 kDa subunit
• RNA polymerase III subunit C3
• RPC62RPC3

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPC62 Antibody (H00010623-B03P)(1)

Publication using H00010623-B03P

• Di F, Nama S, Muhuri M et al. C/EBP? mediates RNA polymerase III-driven transcription of oncomiR-138 in malignant gliomas. Nucleic Acids Res. 2018-01-09 [PMID: 29136251]

Bioinformatics

• Gene Symbol: POLR3C
• Entrez:
  • 10623(Human)
• Uniprot:
  • NP_006459.3(Human)