RPB9 Antibody - BSA Free

Western Blot: RPB9 Antibody [NBP2-93676] - Analysis of extracts of various cell lines, using RPB9 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPB9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPB9 Antibody - BSA Free (NBP2-93676) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human POLR2I (NP_006224.1). MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2I
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RPB9 Antibody - BSA Free

• DNA-directed RNA polymerase II 14.5 kDa polypeptide
• DNA-directed RNA polymerase II subunit I
• DNA-directed RNA polymerase II subunit RPB9
• hRPB14.5
• polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
• polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• RNA polymerase II 14.5 kDa subunit
• RNA polymerase II subunit B9
• RPB14.5
• RPB9

Background

RPB9 encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2I