RPB2 Antibody - BSA Free

Western Blot: RPB2 Antibody [NBP2-93593] - Analysis of extracts of various cell lines, using RPB2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-93593] - Paraffin-embedded rat liver using RPB2 .

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-93593] - Paraffin-embedded mouse liver using RPB2 .

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-93593] - Paraffin-embedded rat brain using RPB2 .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPB2 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1). MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RPB2 Antibody - BSA Free

• DNA-directed RNA polymerase II subunit RPB2
• EC 2.7.7
• EC 2.7.7.6
• hRPB140
• hsRPB2
• POL2RB
• polymerase (RNA) II (DNA directed) polypeptide B (140kD)
• polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• RNA polymerase II subunit 2
• RNA polymerase II subunit B2
• RNA polymerase II subunit2, RNA polymerase II second largest subunit

Background

It is suggested that Rpb2, a 140 kDa protein, plays a role is enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2B