RPB2 Antibody (HL1042) - Azide and BSA Free

Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

• Reactivity: Hu
• Applications: WB
• Clone: HL1042
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RPB2 Antibody (HL1042) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RPB2 Antibody (HL1042) - Azide and BSA Free

• DNA-directed RNA polymerase II subunit RPB2
• EC 2.7.7
• EC 2.7.7.6
• hRPB140
• hsRPB2
• POL2RB
• polymerase (RNA) II (DNA directed) polypeptide B (140kD)
• polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• RNA polymerase II subunit 2
• RNA polymerase II subunit B2
• RNA polymerase II subunit2, RNA polymerase II second largest subunit

Background

It is suggested that Rpb2, a 140 kDa protein, plays a role is enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2B