Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 ...read more
Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500
WB: Predicted MW is 29 kDa. For IP: Use at an assay dependent dilution.
32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
Protein G purified
Alternate Names for RPA2 Antibody (12F3.3)
Replication factor A protein 2
replication protein A 32 kDa subunit
Replication protein A 34 kDa subunit
replication protein A2 (32kD)
replication protein A2, 32kDa
RF-A protein 2
Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK), ataxia telangiectasia-mutated gene (ATM), and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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