ROR gamma t/RORC2/NR1F3 Antibody (1181A) [Alexa Fluor® 488]

Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC9125G) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC9125G) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 1181A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

ROR gamma t/RORC2/NR1F3 Antibody (1181A) [Alexa Fluor® 488] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: E.coli-derived recombinant human ROR gamma t/RORC2/NR1F3
  Met1-Gln100
  Accession # P51449
• Specificity: Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Rorc
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR gamma t/RORC2/NR1F3 Antibody (1181A) [Alexa Fluor® 488]

• IMD42;NR1F3;Nuclear receptor ROR-gamma;RORG;RZRG;RZR-GAMMA;TOR
• ROR gamma t
• RORC2

Background

Retinoic acid-related Orphan Receptor gamma (ROR gamma , TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma , ROR gamma t, has been shown to be specifically expressed in the thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR gamma t/RORC2/NR1F3 Antibody (IC9125G)(1)

Publication using IC9125G

• Lee, Y;Kim, SH;Jeong, H;Kim, KH;Jeon, D;Cho, Y;Lee, D;Nam, KT; Role of Nox4 in mitigating inflammation and fibrosis in dextran sulfate sodium-induced colitis Cellular and molecular gastroenterology and hepatology 2023-05-17 [PMID: 37207801] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Rorc
• Uniprot:
  • P51449()