ROR gamma(t) Plasmid

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ROR gamma(t) Plasmid [NBP2-25279] - Schematic Map of the cloning vector.

Product Details

Summary
Reactivity HuSpecies Glossary
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

ROR gamma(t) Plasmid Summary

Immunogen
Human ROR gamma(t) Plasmid
Specificity
he ROR gamma(t) Plasmid is a mammalian expression vector construct, pCMV/hRORgamma(t), containing coding sequence of human RAR-related orphan receptor gamma(t) (GenBank Accession: NM_001001523).
Gene
RORC

Applications/Dilutions

Application Notes
This product is useful for: Expression of hROR gamma(t) protein in mammalian cells. Induction of IL-17A promoter activity. Total content of 10 ug is enough for one 96-well cell transfection (0.1 ug plasmid per well). Recommended amount for other plate formats: 6-well plate (2 ug/well), 12-well plate (0.8 ug/well) and 24-well plate (0.4 ug/well).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for ROR gamma(t) Plasmid

  • NR1F3
  • Nuclear receptor ROR-gamma
  • Nuclear receptor RZR-gamma
  • Nuclear receptor subfamily 1 group F member 3
  • Retinoid-related orphan receptor-gamma
  • RORC
  • RORG
  • RZRG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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FAQs for ROR gamma(t) Plasmid (NBP2-25279). (Showing 1 - 2 of 2 FAQ).

  1. Our customers has ordered the HEK IL-17A Prom / LUCPorter (tm) Stable Reporter Cell Line (NBP2-26283) that is resistant to puromycin. Because he wanted to transfect the plasmideROR gamma (t) plasmid (NBP2-25279) which has the neomycin resistance, I made a range of neomycin on HEK IL-17A Prom / LUCPorter (tm) Stable Reporter Cell line. The result is surprising because even at a concentration of 4mg/ml which is normally lethal to HEK, cells are still alive. Do these cells contain another plasmid with a neomycin resistance? These are Hek 293 or T 293, or did you have another explanation?
    • The NBP2-26283 cell line was generated from using HEK293 cells from ATCC. Our QC test also confirmed that NBP2-26283 is neomycin resistance. NBP2-25279 assays with NBP2-26283 are based on transient transfection of NBP2-25279 into NBP2-26283. Hence there is no need for neomycin.
  2. Is the neoR gene driven by a functional promoter in mammalian cells? If so, which one?
    • It is SV40 promoter that drives the neomycin gene in NBP2-25279.

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Bioinformatics

Gene Symbol RORC