ROCK2 Antibody

Product Discontinued

ROCK2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 100 of human Rho-associated, coiled-coil containing protein kinase 2 using the number given in entry NP_004841.1 (GeneID 9475).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ROCK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 ug/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ROCK2 Antibody

• coiled-coil-containing protein kinase 2
• EC 2.7.11
• EC 2.7.11.1
• KIAA0619
• p164 ROCK-2
• Rho kinase 2
• rho-associated protein kinase 2
• Rho-associated, coiled-coil containing protein kinase 2
• ROCK2
• ROCK-II
• ROKalpha

Background

The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROCK2 Antibody (NB100-592)(2)

Publications using NB100-592

• Farber MJ, Rizaldy R, HildebrJD et al. Shroom2 regulates contractility to control endothelial morphogenesis. Mol Biol Cell 2011-03-01 [PMID: 21248203]
• Mattaliano MD, Wooters J, Shih HH et al. ROCK2 associates with lectin-like oxidized LDL receptor-1 and mediates oxidized LDL-induced IL-8 production. Am J Physiol Cell Physiol 2010-05-01 [PMID: 20181930]

Bioinformatics

• Gene Symbol: ROCK2
• Entrez:
  • 9475(Human)
• Uniprot:
  • NP_004841.1(Human)