ROCK2 Antibody (2A4) - Azide and BSA Free

Western Blot: ROCK2 Antibody (2A4) [H00009475-M01] - ROCK2 monoclonal antibody (M01), clone 2A4 Analysis of ROCK2 expression in HeLa.

Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (2A4) [H00009475-M01] - Analysis of monoclonal antibody to ROCK2 on HeLa cell. Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ROCK2 Antibody (2A4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ROCK2 Antibody (2A4) - Azide and BSA Free (H00009475-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ROCK2 (NP_004841, 1279 a.a. ~ 1388 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
• Localization: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
• Specificity: ROCK2 - Rho-associated, coiled-coil containing protein kinase 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROCK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ROCK2 Antibody (2A4) - Azide and BSA Free

• coiled-coil-containing protein kinase 2
• EC 2.7.11
• EC 2.7.11.1
• KIAA0619
• p164 ROCK-2
• Rho kinase 2
• rho-associated protein kinase 2
• Rho-associated, coiled-coil containing protein kinase 2
• ROCK2
• ROCK-II
• ROKalpha

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ROCK2
• Entrez:
  • 9475(Human)
• OMIM:
  • 604002(Human)
• Uniprot:
  • NP_004841(Human)