ROCK1 Antibody (701118)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROCK1 Monoclonal Antibody (Catalog # MAB4590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROCK1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 701118
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ROCK1 Antibody (701118) Summary

• Immunogen: E. coli-derived recombinant human ROCK1
  Ala629-Thr829
  Accession # Q13464
• Specificity: Detects human ROCK1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ROCK2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROCK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROCK1 Antibody (701118)

• EC 2.7.11
• EC 2.7.11.1
• MGC131603
• MGC43611
• p160 ROCK-1
• p160ROCK
• p160-ROCK
• PRO0435
• Renal carcinoma antigen NY-REN-35
• Rho kinase
• rho-associated protein kinase 1
• Rho-associated, coiled-coil containing protein kinase 1
• Rho-associated, coiled-coil-containing protein kinase 1
• ROCK1
• ROK beta

Background

ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis, which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).

1. Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
2. Zhang, C. et al. (2006) FASEB J. 20:916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROCK1 Antibody (MAB4590)(2)

Publications using MAB4590

• Kamble, D;Ropa, JP;Kamocka, MM;Qi, Y;Imperiale, N;Singh, P; Neuropeptide Y deficiency in the bone marrow drives hematopoietic stem and progenitor cell aging bioRxiv : the preprint server for biology 2026-02-20 [PMID: 41756904] (Mouse)
• Lee, E;Chan, SL;Lee, Y;Polacheck, WJ;Kwak, S;Wen, A;Nguyen, DT;Kutys, ML;Alimperti, S;Kolarzyk, AM;Kwak, TJ;Eyckmans, J;Bielenberg, DR;Chen, H;Chen, CS; A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex Proceedings of the National Academy of Sciences of the United States of America 2023-10-10 [PMID: 37782785] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ROCK1
• Uniprot:
  • Q13464()