ROCK1 Antibody (12M03)

Western Blot: cleaved ROCK1 Antibody (12M03) [NBP1-46006] - Jurkat T cells (Lane A:10 ug, Lane B: 25 ug, Lane C: 50 ug) stained with cleaved ROCK1 antibody.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 12M03
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ROCK1 Antibody (12M03) Summary

• Immunogen: Synthetic peptide corresponding to the caspase-3 cleavage site.
• Specificity: The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROCK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 4 ug/ml

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 1% BSA, 50% Glycerol
• Preservative: 0.02% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for ROCK1 Antibody (12M03)

• EC 2.7.11
• EC 2.7.11.1
• MGC131603
• MGC43611
• p160 ROCK-1
• p160ROCK
• p160-ROCK
• PRO0435
• Renal carcinoma antigen NY-REN-35
• Rho kinase
• rho-associated protein kinase 1
• Rho-associated, coiled-coil containing protein kinase 1
• Rho-associated, coiled-coil-containing protein kinase 1
• ROCK1
• ROK beta

Background

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ROCK1
• Entrez:
  • 6093(Human)
• Uniprot:
  • Q13464()