![Western Blot: ROBO3 Antibody (2H1) [H00064221-M02] - Analysis of ROBO3 expression in transfected 293T cell line by ROBO3 monoclonal antibody (M02), clone 2H1.Lane 1: ROBO3 transfected lysate (Predicted MW: 16.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ROBO3-Antibody-2H1-Western-Blot-H00064221-M02-img0002.jpg "Western Blot: ROBO3 Antibody (2H1) [H00064221-M02] - Analysis of ROBO3 expression in transfected 293T cell line by ROBO3 monoclonal antibody (M02), clone 2H1.Lane 1: ROBO3 transfected lysate (Predicted MW: 16.1 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 2H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | ROBO3 (AAH08623, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSQSRSQSQRPGQKRREEPR |
| Specificity | Reacts with roundabout, axon guidance receptor, homolog 3 (Drosophila). |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ROBO3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is reactive against recombinant protein in western blot and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ROBO3 Antibody (H00064221-M02)Find related products by research area.
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![Sandwich ELISA: ROBO3 Antibody (2H1) [H00064221-M02] - Detection limit for recombinant GST tagged ROBO3 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ROBO3-Antibody-2H1-Sandwich-ELISA-H00064221-M02-img0001.jpg "Sandwich ELISA: ROBO3 Antibody (2H1) [H00064221-M02] - Detection limit for recombinant GST tagged ROBO3 is 0.1 ng/ml as a capture antibody.")
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![Western Blot ROBO4 Antibody [Unconjugated]](https://images.novusbio.com/images/af2366_human-robo4-affinity-purified-polyclonal-ab-western-blot-12122025929454.jpg)
![Western Blot ROBO4 Antibody [Unconjugated]](https://images.novusbio.com/images/af2366_human-robo4-affinity-purified-polyclonal-ab-western-blot-121220259305221.jpg)
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
