RNPC3 Overexpression Lysate

Western Blot: RNPC3 Overexpression Lysate (Adult Normal) [NBL1-15469] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNPC3.

• Reactivity: Hu
• Applications: WB

RNPC3 Overexpression Lysate Summary

• Description: Transient overexpression lysate of RNA-binding region (RNP1, RRM) containing 3 (RNPC3) The lysate was created in HEK293T cells, using Plasmid ID RC202319 and based on accession number NM_017619. The protein contains a C-MYC/DDK Tag.
• Gene: RNPC3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 58.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNPC3 Overexpression Lysate

• FLJ20008
• FLJ25070
• KIAA183
• KIAA1839
• RBM40U11/U12 snRNP 65 kDa protein
• RNA recognition protein
• RNA-binding motif protein 40
• RNA-binding protein 40
• RNA-binding region (RNP1, RRM) containing 3
• RNA-binding region-containing protein 3
• RNP
• U11/U12 small nuclear ribonucleoprotein 65 kDa protein
• U11/U12 snRNP 65K
• U11/U12-65K

Background

Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RNPC3