RNMT [p Tyr1235] Antibody (NL8)

Western Blot: RNMT [p Tyr1235] Antibody (NL8) [NBP2-29685] - Untreated (Lane -) and lambda-phosphatase treated (Lane +) RIPA lysate from A431 cells co-expressing Met/HGFR and HGF were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-Met (Tyr1234/1235), clone NL8.Arrow indicates phospho-Met (~140 kDa).

Product Discontinued

RNMT [p Tyr1235] Antibody (NL8) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH-conjugated, synthetic peptide in which pYpY corresponds to phosphorylated amino acids 1234 and 1234 of human Met/HGFR.
• Modification: p Tyr1235
• Specificity: Recognizes phosphorylated Met, Mr 140 kDa. Cross-reacts with phosphorylated EGFR.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RNMT
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Relevant Entrez Gene Numbers: NP_000236.2

Alternate Names for RNMT [p Tyr1235] Antibody (NL8)

• DKFZp686H1252
• EC 2.1.1.56
• hcm1p
• hCMT1
• hCMT1c
• hMet
• KIAA0398
• mRNA (guanine-7-)methyltransferase
• mRNA (guanine-N(7)-)-methyltransferase
• mRNA cap guanine-N7 methyltransferase
• mRNA cap methyltransferase
• RG7MT1MET
• RNA (guanine-7-) methyltransferase

Background

The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNMT
• Uniprot:
  • P08581()