RNF7 Antibody

Images

 
Western Blot: RNF7 Antibody [NBP1-46159] - Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RNF7 Antibody Summary

Immunogen
Peptide with sequence DACLRCQAENKQE corresponding to internal region according to NP_055060.1.
Specificity
This antibody is expected to recognize isoform 1 (NP_055060.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNF7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 11 kDa band observed in human human heart lysates (calculated MW of 12.7 kDa band according to NP_055060.1). An additional band of 28 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-46159.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF7 Antibody

  • CKBBP1zinc RING finger protein SAG
  • CKII beta-binding protein 1
  • RBX2
  • Regulator of cullins 2
  • ring finger protein 7rbx2
  • ROC2RING-box protein 2
  • SAGsensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
  • Sensitive to apoptosis gene protein

Background

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNF7 Antibody (NBP1-46159)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol RNF7
Entrez
Uniprot