RNF40 Antibody

Images

 
Western Blot: RNF40 Antibody [NBP1-53086] - Antibody Titration: 1.25ug/ml Positive Control: 293T cell lysate
Immunohistochemistry: RNF40 Antibody [NBP1-53086] - SampleType : Adult mouse cerebellum (l0x) section Dilution : 1: 100 Secondary Antibody : Donkey Anti-rabbit (568 nm) Secondary Antibody Dilution : 1: 500 Color/Signal ...read more
Immunohistochemistry: RNF40 Antibody [NBP1-53086] - Human Muscle

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RNF40 Antibody Summary

Immunogen
Synthetic peptides corresponding to RNF40(ring finger protein 40) The peptide sequence was selected from the C terminal of RNF40. Peptide sequence KLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELT.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF40
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against RNF40 and was validated on Western Blot and immunohistochemistry-P
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for RNF40 Antibody

  • 95 kDa retinoblastoma-associated protein
  • BRE1-B
  • BRE1BMGC13051
  • EC 6.3.2,95 kDa retinoblastoma protein binding protein
  • EC 6.3.2.-
  • KIAA0661BRE1 E3 ubiquitin ligase homolog B
  • Rb-associated protein
  • RBP95E3 ubiquitin-protein ligase BRE1B
  • ring finger protein 40DKFZp686K191
  • STARING

Background

RNF40 contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RNF40
Uniprot