RNF40 Antibody

Western Blot: RNF40 Antibody [NB100-2247] - Detection of Human RNF40 on HeLa whole cell lysate using NB100-2247. RNF40 was also immunoprecipitated using rabbit anti-RNF40 antibodies NB100-2246 and NB100-2248.

Immunohistochemistry-Paraffin: RNF40 Antibody [NB100-2247] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RNF40 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RNF40 Antibody Summary

• Description: Novus Biologicals Rabbit RNF40 Antibody (NB100-2247) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-RNF40 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 540 and 590 of human Ring Finger 40 using the numbering given in entry NP_055586.1 (GeneID 9810).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF40
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  RNF40 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF40 Antibody

• 95 kDa retinoblastoma-associated protein
• BRE1-B
• BRE1BMGC13051
• EC 6.3.2,95 kDa retinoblastoma protein binding protein
• EC 6.3.2.-
• KIAA0661BRE1 E3 ubiquitin ligase homolog B
• Rb-associated protein
• RBP95E3 ubiquitin-protein ligase BRE1B
• ring finger protein 40DKFZp686K191
• STARING

Background

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 40 (RNF40) is a member of the RING type E3 ubiquitin ligases. RNF40 forms a complex with RNF20 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RN40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF40 include E3 ubiquitin-protein ligase BRE1B, BRE1-B, 95kDa retinoblastoma-associated protein, RBP95, STARING, KIAA0661, MGC13051, and DKFZp686K191.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF40 Antibody (NB100-2247)(1)

Publication using NB100-2247

• Lyu Y, Yang Y, Talwar V et al Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes Cell Rep 2024-03-22 [PMID: 38517892] (WB, Human)

Bioinformatics

• Gene Symbol: RNF40
• Entrez:
  • 9810(Human)
• Uniprot:
  • NP_055586.1(Human)