RNF4 Antibody [Unconjugated]

Western blot shows lysates of GM00719D human ataxia telangiectasia cell line either mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour, BaF3 mouse pro-B cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RNF4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RNF4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

RNF4 was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

RNF4 Antibody [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human RNF4
  Met1-Ile190
  Accession # P78317
• Specificity: Detects human RNF4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RNF4 Antibody [Unconjugated]

• E3 ubiquitin ligase RNF4
• EC 6.3.2.-
• Protein SNURF
• RES4-26
• ring finger protein 4SNURFSLX5
• RNF4
• SLX5
• Small nuclear ring finger protein
• SNURF

Background

RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.

1. Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
2. Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF4 Antibody (AF7964)(7)

Publications using AF7964

• Rodríguez Pérez, F;Natwick, D;Schiff, L;McSwiggen, D;Heckert, A;Huey, M;Morrison, H;Loo, M;Miranda, RG;Filbin, J;Ortega, J;Van Buren, K;Murnock, D;Tao, A;Butler, R;Cheng, K;Tarvestad, W;Zhang, Z;Gonzalez, E;Miller, RM;Kelly, M;Tang, Y;Ho, J;Anderson, D;Bashore, C;Basham, S; WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis Nature communications 2024-07-18 [PMID: 39025847] (Western Blot, Human)
• Zhang, H;Xiong, Y;Sun, Y;Park, JM;Su, D;Feng, X;Keast, S;Tang, M;Huang, M;Wang, C;Srivastava, M;Yang, C;Zhu, D;Chen, Z;Li, S;Yin, L;Pommier, Y;Chen, J; RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons Science advances 2023-12-08 [PMID: 38055811] (Western Blot, Human)
• Krawic, C;Luczak, MW;Valiente, S;Zhitkovich, A; Atypical genotoxicity of carcinogenic nickel(II): linkage to dNTP biosynthesis, DNA-incorporated rNMPs and impaired repair of TOP1-DNA crosslinks The Journal of biological chemistry 2023-10-25 [PMID: 37890780] (Western Blot, Human)
• Chang, YC;Lin, K;Baxley, RM;Durrett, W;Wang, L;Stojkova, O;Billmann, M;Ward, H;Myers, CL;Bielinsky, AK; RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication Open biology 2023-08-01 [PMID: 37607592] (Western Blot, Human)
• P Weickert, HY Li, MJ Götz, S Dürauer, D Yaneva, S Zhao, J Cordes, AC Acampora, I Forne, A Imhof, J Stingele SPRTN patient variants cause global-genome DNA-protein crosslink repair defects Nature Communications, 2023-01-21;14(1):352. 2023-01-21 [PMID: 36681662] (Western Blots, Human)
• MJ Young, YC Chen, SA Wang, HP Chang, WB Yang, CC Lee, CY Liu, YL Tseng, YC Wang, HS Sun, WC Chang, JJ Hung Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression Journal of Biomedical Science, 2022-01-17;29(1):3. 2022-01-17 [PMID: 35034634] (Western Blot, Xenograft)
• Z Sha, T Blyszcz, R González-P, ACO Vertegaal, AL Goldberg Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies J. Biol. Chem., 2019-07-08;0(0):. 2019-07-08 [PMID: 31285264] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P78317(Human)