RNF23 Antibody

Images

 
Immunohistochemistry-Paraffin: RNF23 Antibody [NB100-1187] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RNF23 Antibody Summary

Immunogen
Peptide with sequence C-NAAPLTIRPPTDWE corresponding to C-Terminus according to NP_067076.2, NP_742013.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_067076.2 and NP_742013.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM39
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 49 kDa band in human testis and rat testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4 kDa band according to NP_NP_742013.1 and of 59.7 kDa band according to NP_067076.2. The 49 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Uterus.
Positive Control
RNF23 Lysate (NBL1-17295)
Publications
Read Publication using NB100-1187.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, and Porcine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RNF23 Antibody

  • MGC32984
  • RING finger protein 23RNF23TFPtripartite motif-containing 39
  • Testis-abundant finger protein
  • TRIM39B
  • tripartite motif containing 39
  • tripartite motif-containing protein 39

Background

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1187
Species: Hu
Applications: IHC-P, PEP-ELISA

Publications for RNF23 Antibody (NB100-1187)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM39
Entrez
Uniprot