RNF23 Antibody

Immunohistochemistry-Paraffin: RNF23 Antibody [NB100-1187] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RNF23 Antibody Summary

• Immunogen: Peptide with sequence C-NAAPLTIRPPTDWE corresponding to C-Terminus according to NP_067076.2, NP_742013.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_067076.2 and NP_742013.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM39
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 49 kDa band in human testis and rat testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4 kDa band according to NP_NP_742013.1 and of 59.7 kDa band according to NP_067076.2. The 49 kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Uterus.
• Control:

  RNF23 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF23 Antibody

• MGC32984
• RING finger protein 23RNF23TFPtripartite motif-containing 39
• Testis-abundant finger protein
• TRIM39B
• tripartite motif containing 39
• tripartite motif-containing protein 39

Background

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF23 Antibody (NB100-1187)(1)

Publication using NB100-1187

• Orimo A, Yamagishi T, Tominaga N et al. Molecular cloning of testis-abundant finger Protein/Ring finger protein 23 (RNF23), a novel RING-B box-coiled coil-B30.2 protein on the class I region of the human MHC. Biochem Biophys Res Commun 2000-09-16 [PMID: 11006080]

Bioinformatics

• Gene Symbol: TRIM39
• Entrez:
  • 56658(Human)
  • 79263(Mouse)
• Uniprot:
  • NP_067076(Human)