RNF168 Antibody - BSA Free

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - RNF168 Antibody [NBP1-76324] - Western blot analysis showing the expression of BMI1, RING1A, RING1B, EZH2, gamma-H2AX, Ub-H2A, p-CHK2, p-ATM, RNF8, RNF168, MEL18, p53BP, BRCA1 proteins in MDAMB-231cells transfected with miR-15a, miR-16 or both miR-15a/16 under etoposide-induced DNA damage conditions. Actin served as a gel loading control. Blots were cropped to enhance the representation. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-02800-2), licensed under a CC-BY license.

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - RNF168 Antibody [NBP1-76324] - Human brain tissue lysate with RNF168 antibody at 1 ug/mL.

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - Ectopic expression of miR-15a, miR-16 sensitizes breast cancer cells to DNA damage through BMI1 dependent ubiquitination pathway. Western blot analysis showing the expression of BMI1, RING1A, RING1B, EZH2, gamma -H2AX, Ub-H2A, p-CHK2, p-ATM, RNF8, RNF168, MEL18, p53BP, BRCA1 proteins in MDAMB-231 cells transfected with miR-15a, miR-16 or both miR-15a/16 under etoposide-induced DNA damage conditions. Actin served as a gel loading control. Blots were cropped to enhance the representation (A,B). Immunofluorescence data showing accumulation of BMI1, ϒ-H2AX (C) RING1B (D) & Ub-H2A (E) p53BP (F) in MDAMB-231 cells transfected with miR-15a miR-16 or both miR-15a/16 under etoposide- treated conditions. Bar indicates 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28655885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RNF168 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RNF168 Antibody - BSA Free (NBP1-76324) is a polyclonal antibody validated for use in WB and ELISA. Anti-RNF168 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168. Amino Acid Squence: KEVDKEQMVPNRQKGSPD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF168
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
• Control Peptide:

  RNF168 Peptide (NBP1-76324PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Bovine (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RNF168 Antibody - BSA Free

• E3 ubiquitin-protein ligase RNF168
• EC 6.3.2
• EC 6.3.2.-
• FLJ39749
• ring finger protein 168FLJ35794
• RNF168

Background

RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF168 Antibody (NBP1-76324)(1)

Publication using NBP1-76324

• Patel N, Garikapati KR, Pandita RK et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin Sci Rep 2017-06-27 [PMID: 28655885] (WB, Human)

Bioinformatics

• Gene Symbol: RNF168
• Uniprot:
  • NP_689830(Human)