RNF111 Antibody

Immunocytochemistry/Immunofluorescence: RNF111 Antibody [NBP1-94139] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: RNF111 Antibody [NBP1-94139] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Product Discontinued

RNF111 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TLKVDMKSEIPSDAPKTQESLKGILLHPEPIGAAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGPSYVQNCVKENQGI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF111
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%). Human reactivity reported in scientific literature (PMID: 26522722).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNF111 Antibody

• ARK
• Arkadia
• DKFZp313E0731
• DKFZp761D081
• E3 ubiquitin-protein ligase Arkadia
• EC 6.3.2
• EC 6.3.2.-
• FLJ38008
• ring finger protein 111DKFZp686H1966

Background

RNF111 is encoded by this gene contains a RING finger domain, a motif known to be involved in protein-protein and protein-DNA interactions. The mouse counterpart of this gene (Rnf111/arkadia) has been shown to genetically interact with the transforming growth factor (TGF) beta-like factor Nodal, and act as a modulator of the nodal signaling cascade, which is essential for the induction of mesoderm during embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF111 Antibody (NBP1-94139)(1)

Publication using NBP1-94139

• A Mizutani, D Koinuma, H Seimiya et al. The Arkadia-ESRP2 axis suppresses tumor progression: analyses in clear-cell renal cell carcinoma. Oncogene 2015-11-02 [PMID: 26522722] (WB, Human)

Bioinformatics

• Gene Symbol: RNF111