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RNF10 Antibody

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Western Blot: RNF10 Antibody [NBP2-93965] - Analysis of extracts of various cell lines, using RNF10 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
Western Blot: RNF10 Antibody [NBP2-93965] - Analysis of extracts of various cell lines, using RNF10 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RNF10 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 552-811 of human RNF10 (NP_055683.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF10
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RNF10 Antibody

  • KIAA0262RIE2MGC126758
  • MGC126764
  • ring finger protein 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00004223-M03
Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in transfected 293T cell line by MEOX2 monoclonal antibody (M03), clone 6A5. Lane 1: MEOX2 transfected lysatE (33 KDa). Lane 2: Non-transfected lysate.
ELISA: MEOX 2 Antibody (6A5) [H00004223-M03] - Detection limit for recombinant GST tagged MEOX2 is approximately 0.3ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of monoclonal antibody to MEOX2 on HeLa cell. Antibody concentration 10 ug/ml
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WDR42A Antibody
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Immunohistochemistry-Paraffin: WDR42A Antibody [NBP1-92582] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: WDR42A Antibody [NBP1-92582] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: WDR42A Antibody [NBP1-92582] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Species: Hu
Applications: IHC, IHC-P, WB
MYST1 Antibody
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Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
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PCYT1A Antibody
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Immunocytochemistry/Immunofluorescence: PCYT1A Antibody [NBP1-84366] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: PCYT1A Antibody [NBP1-84366] - Analysis in human cell line EFO-21.
Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP1-84366] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
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RNF111 Antibody
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ELISA: RNF111 Antibody (1C4) [H00054778-M05] - Detection limit for recombinant GST tagged RNF111 is 0.03 ng/ml as a capture antibody.
Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Western blot analysis of RNF111 over-expressed 293 cell line, cotransfected with RNF111 Validated Chimera RNAi or non-transfected control. Blot probed with H00054778-M05. GAPDH (36.1 kDa) used as loading control.
Immunocytochemistry/Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of monoclonal antibody to RNF111 on HeLa cell. Antibody concentration 10 ug/ml
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Immunohistochemistry-Paraffin: TEL2 Antibody [NBP1-81761] - Staining of human urinary bladder shows cytoplasmic and nuclear positivity in urothelial cells.
Western Blot: TEL2 Antibody [NBP1-81761] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: TEL2 Antibody [NBP1-81761] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol.
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Species: Hu
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beta-Glucuronidase/GUS
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Immunocytochemistry/Immunofluorescence: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - Analysis in human liver and skeletal muscle tissues using NBP1-87511 antibody. Corresponding GUSB RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - Staining of human liver shows strong positivity in lysosomes in hepatocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Paxillin Antibody
(SY23-02)
NBP2-67427
Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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JIK Antibody
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ELISA: RhoF Antibody (1D4B6) [NBP2-52553] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Flow Cytometry: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).
Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.
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EIF3B Antibody
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Immunohistochemistry-Paraffin: EIF3B Antibody [NB100-93302] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiEIF3S9/eIF3B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: EIF3B Antibody [NB100-93302] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3B antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: EIF3B Antibody [NB100-93302] - Detection of human eIF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3B antibody NB100-93302 used for IP at 3 ug per reaction. eIF3B was also immunoprecipitated by another rabbit anti-eIF3B antibody. For blotting immunoprecipitated eIF3B, NB100-93302 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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JAB1 Antibody
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Immunohistochemistry: JAB1 Antibody (2A10) [NB120-495]
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody [2A10.8] Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE JAB1 Antibody diluted at 1:10000
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Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
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Cyclophilin B
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Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.
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TTC4 Antibody
NBP2-59139
Western Blot: TTC4 Antibody [NBP2-59139] - Detection of human TTC4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TTC4 antibody NBP2-59139 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: TTC4 Antibody [NBP2-59139] - Detection of human TTC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TTC4 antibody NBP2-59139 used for IP at 20 ul per reaction. TTC4 was also immunoprecipitated by another rabbit anti-TTC4 antibody. For blotting immunoprecipitated TTC4, NBP2-59139 was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Applications: IP, WB
Calreticulin
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Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.
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Species: Bv, Ha, Hu, Mu, Pm, Rt
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Annexin V
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NBP2-29373
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Flow cytometry image submitted by a verified customer review.
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DENR Antibody
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Western Blot: DENR Antibody [NBP2-13913] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: DENR Antibody [NBP2-13913] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Western Blot: DENR Antibody [NBP2-13913] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-159
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RNF10 Antibody
NBP2-93965
RNF10 Antibody
Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol RNF10