RNASEH2A Antibody - BSA Free

Western Blot: RNASEH2A Antibody [NBP1-76981] - HeLa cell lysate with RNAse H2A antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981] - Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981] - Immunofluorescence of RNase H2A in Hela cells with RNAse H2A antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RNASEH2A Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A. Amino Acid Squence: LDTDYGSGYPNDPKTKA
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNASEH2A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-4 ug/ml
  • Immunoprecipitation
  • Western Blot 1 ug/ml
• Application Notes: IP reactivity reported in (PMID: 26106861).
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  RNASEH2A Peptide (NBP1-76981PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RNASEH2A Antibody - BSA Free

• AGS4RNase H2 subunit A
• Aicardi-Goutieres syndrome 4 protein
• Aicardi-Goutieres syndrome 4
• JUNB
• ribonuclease H2 subunit A
• ribonuclease H2, large subunit
• ribonuclease H2, subunit A
• Ribonuclease HI large subunit
• Ribonuclease HI subunit A
• ribonuclease HI, large subunit
• RNase H(35)
• RNASEHIEC 3.1.26.4
• RNHIARNase HI large subunit
• RNHL

Background

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNASEH2A Antibody (NBP1-76981)(3)

Publications using NBP1-76981

• Porter H, Li Y, Neguembor MV et al. Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading eLife 2023-04-03 [PMID: 37010886] (Immunoprecipitation, Human)
• Wang C, Wang G, Feng X et al. Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition Oncogene 2018-12-07 [PMID: 30532030] (WB, Human)
• Tresini M, Warmerdam DO, Kolovos P et al. The core spliceosome as target and effector of non-canonical ATM signalling. Nature 2015-07-02 [PMID: 26106861] (IP, Human)

Bioinformatics

• Gene Symbol: RNASEH2A
• Uniprot:
  • O75792(Human)