RNA Polymerase II/POLR2A [p Ser1619] Antibody

Product Discontinued

RNA Polymerase II/POLR2A [p Ser1619] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-S^P-P-S). (C-terminal)
• Modification: p Ser1619
• Specificity: POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at Human Ser1619, Mouse Ser1619 or Rat Ser1598.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human, Mouse and Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNA Polymerase II/POLR2A [p Ser1619] Antibody

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNA Polymerase II/POLR2A Antibody (NBP2-04145)(1)

Publication using NBP2-04145

• Olaf R Mook. Mol. Cancer Ther; 6: 833 - 843. [PMID: 17363479]

Bioinformatics

• Gene Symbol: POLR2A
• OMIM:
  • 180660(Human)
• Uniprot:
  • P24928(Human)