RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51)

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti- Phospho-POLR2A (S2) antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2: MCF-7

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Staining Phospho-POLR2A (S2) in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.

Flow Cytometry: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Analysis of Hela cells with Phospho-POLR2A (S2) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibod

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Staining Phospho-POLR2A (S2) in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) [NBP2-66800] - Analysis of paraffin-embedded mouse testis tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.

Product Discontinued

RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser2 of Human RNA Polymerase II/POLR2A aa 1590-1630 / 1970. (SwissProt: P24928 Human; SwissProt: P08775 Mouse; Entrez Gene: 363633 Rat)
• Modification: p Ser2
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLR2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RNA Polymerase II/POLR2A [p Ser2] Antibody (JM11-51)

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2A