RNA Polymerase II/POLR2A Antibody (C.15100055)

Western Blot: RNA Polymerase II/POLR2A Antibody (C.15100055) [NBP2-59209] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A Antibody (C.15100055) [NBP2-59209] - ChIP assays were performed using human HeLa cells, the antibody against Pol II and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 uL of antibody per ChIP experiment was analyzed. IgG (2 ug) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Product Discontinued

RNA Polymerase II/POLR2A Antibody (C.15100055) Summary

• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2A
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2 uL/IP
  • Chromatin Immunoprecipitation Sequencing 2 uL/IP
  • Knockdown Validated
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites Fluid
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for RNA Polymerase II/POLR2A Antibody (C.15100055)

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2A