RNA Helicase A Antibody

Western Blot: RNA Helicase A Antibody [NBP1-28915] - (1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RNA Helicase A Antibody Summary

• Description: Novus Biologicals Goat RNA Helicase A Antibody (NBP1-28915) is a polyclonal antibody validated for use in WB and ELISA. Anti-RNA Helicase A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence TEGRNALIHKSSVNC corresponding to internal region according to NP_001348.2.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DHX9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 140 kDa band observed in HeLa nuclear lysates (calculated MW of 141 kDa band according to NP_001348.2).
• Theoretical MW: 141 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNA Helicase A Antibody

• DDX9
• DDX9ATP-dependent RNA helicase A
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin)
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
• DEAH (Asp-Glu-Ala-His) box polypeptide 9
• DEAH box protein 9
• DExH-box helicase 9
• DHX9
• EC 3.6.1
• EC 3.6.1.15
• EC 3.6.4.13
• FLJ17406
• Leukophysin
• LKP
• NDH II
• NDH2
• NDHII
• Nuclear DNA helicase II
• RHA
• RNA Helicase A

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNA Helicase A Antibody (NBP1-28915)(1)

Publication using NBP1-28915

• Toretsky JA, Erkizan V, Levenson A et al. Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A. Cancer Res 2006-06-01 [PMID: 16740692]

Bioinformatics

• Gene Symbol: DHX9
• Entrez:
  • 1660(Human)
  • 13211(Mouse)
  • 304859(Rat)
• Uniprot:
  • Q08211(Human)