RIG-I Overexpression Lysate (Native)

Western Blot: DDX58 Overexpression Lysate (Adult Normal) [NBL1-09808] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX58.

Western Blot: DDX58 Overexpression Lysate (Adult Normal) [NBL1-09808] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DDX58.

• Reactivity: Hu
• Applications: WB

RIG-I Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC217615 and based on accession number NM_014314. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.
• Gene: DDX58

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 106.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for RIG-I

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RIG-I Overexpression Lysate (Native)

• DDX58
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• DEAD box protein 58
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• DKFZp686N19181
• EC 3.6.4.13
• FLJ13599
• probable ATP-dependent RNA helicase DDX58
• retinoic acid inducible gene I
• Retinoic acid-inducible gene 1 protein
• Retinoic acid-inducible gene I protein
• RIG-1
• RIGI
• RIG-I
• RIG-IDKFZp434J1111
• RNA helicase RIG-I

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for RIG-I Lysate (NBL1-09808)(1)

Publication using NBL1-09808

• de Rivero Vaccari JP, Brand FJ, Sedaghat C et al. RIG-1 receptor expression in the pathology of Alzheimer's disease. J Neuroinflammation 2014 Apr 08 [PMID: 24694234] (WB)

Bioinformatics

• Gene Symbol: DDX58
• Entrez:
  • 23586(Human)
  • 230073(Mouse)