Western Blot: DDX58 Overexpression Lysate (Adult Normal) [NBL1-09808] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX58.
Western Blot: DDX58 Overexpression Lysate (Adult Normal) [NBL1-09808] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DDX58.
Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) The lysate was created in HEK293T cells, using Plasmid ID RC217615 and based on accession number NM_014314. The protein contains a C-MYC/DDK Tag.
Gene
DDX58
Applications/Dilutions
Application Notes
This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
106.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for RIG-I Overexpression Lysate
DDX58
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DEAD box protein 58
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
DKFZp686N19181
EC 3.6.4.13
FLJ13599
probable ATP-dependent RNA helicase DDX58
retinoic acid inducible gene I
Retinoic acid-inducible gene 1 protein
Retinoic acid-inducible gene I protein
RIG-1
RIGI
RIG-I
RIG-IDKFZp434J1111
RNA helicase RIG-I
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.