RIG-I Antibody (OTI6C1)

Western Blot: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIG-I.

Immunocytochemistry/Immunofluorescence: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RIG-I.

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of extracts (10ug) from COS7 cell line.

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: RIG-I Antibody (OTI6C1) [NBP2-45678] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI6C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RIG-I Antibody (OTI6C1) Summary

• Immunogen: Full length human recombinant protein of human DDX58(NP_055129) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIGI
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:4000
• Theoretical MW: 106.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RIG-I Antibody (OTI6C1)

• DDX58
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• DEAD box protein 58
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
• DKFZp686N19181
• EC 3.6.4.13
• FLJ13599
• probable ATP-dependent RNA helicase DDX58
• retinoic acid inducible gene I
• Retinoic acid-inducible gene 1 protein
• Retinoic acid-inducible gene I protein
• RIG-1
• RIGI
• RIG-I
• RIG-IDKFZp434J1111
• RNA helicase RIG-I

Background

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIGI