RIG-I Antibody (4G1B6)

Images

 
Western Blot: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis using DDX58 mAb against human DDX58 (AA: 789-925) recombinant protein. (Expected MW is 42 kDa)
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of MCF-7 cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of Hela cells using DDX58 mouse mAb (green) and negative control (red).
Western Blot: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis using DDX58 mAb against HEK293 (1) and DDX58 (AA: 789-925)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of Hela cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
ELISA: RIG-I Antibody (4G1B6) [NBP2-61849] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
4G1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RIG-I Antibody (4G1B6) Summary

Immunogen
Purified recombinant fragment of human DDX58 (AA: 789-925) expressed in E. Coli.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
DDX58
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Theoretical MW
106.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for RIG-I Antibody (4G1B6)

  • DDX58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEAD box protein 58
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • DKFZp686N19181
  • EC 3.6.4.13
  • FLJ13599
  • probable ATP-dependent RNA helicase DDX58
  • retinoic acid inducible gene I
  • Retinoic acid-inducible gene 1 protein
  • Retinoic acid-inducible gene I protein
  • RIG-1
  • RIGI
  • RIG-I
  • RIG-IDKFZp434J1111
  • RNA helicase RIG-I

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DDX58