RIG-I Antibody (2M6F10)

Images

 
Western Blot: RIG-I Antibody (2M6F10) [NBP2-27109] - Analysis of RIG-I in Jurkat cell lysate in the A) absence and B) presence of immunizing peptide and C) HEK 293 cell lysate using NBP2-27109 at 10 ug/ml.
Immunohistochemistry: RIG-I Antibody (2M6F10) [NBP2-27109] - Analysis of RIG-I in formalin-fixed, paraffin-embedded human kidney using this antibody at 5 ug/ml.
Simple Western: RIG-I Antibody (2M6F10) [NBP2-27109] - Simple Western lane view shows a specific band for RIG-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-27109
    • Availability
      Product Discontinued

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RIG-I Antibody (2M6F10) Summary

Immunogen
A portion of amino acid 1-50 of human RIG-I was used as the immunogen. 31 PWFREEEVQYIQAEkNNkG 49
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDX58
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5-10ug/ml~
  • Simple Western 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publications using
NBP2-27109 in the following applications:

  • WB
    2 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27463126). The amino acid sequence used as immunogen is 100% homologous in human, chimp, orangutan and rhesus monkey, 85% homologous in pig, 79% homologous in mouse and horse and 73% homologous in rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RIG-I Antibody (2M6F10)

  • DDX58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEAD box protein 58
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • DKFZp686N19181
  • EC 3.6.4.13
  • FLJ13599
  • probable ATP-dependent RNA helicase DDX58
  • retinoic acid inducible gene I
  • Retinoic acid-inducible gene 1 protein
  • Retinoic acid-inducible gene I protein
  • RIG-1
  • RIGI
  • RIG-I
  • RIG-IDKFZp434J1111
  • RNA helicase RIG-I

Background

RIG-I, an RNA helicase of the DEAD/H box family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure and in innate immune defense against viruses. It acts as a specific sensor for dsRNA, which is absent in uninfected cells but known to be accumulated in virus-infected cells. It contains two CARD domains, one helicase ATP-binding domain and the other helicase C-terminal domain. The CARD domains actually inhibit the activity of RIG-I in normal cell, but once the cell gets infected, RIG I directly binds uncapped 5-prime-triphosphate RNA present in viruses triggering an immune response signal. The signaling cascade involves MAVS/IPS1 which induces the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5) during viral replication through activation of NF-kappa-B, IRF3 and IRF7. The ability of RIG I to sense 5-prime-phosphorylated RNA evolved in the innate immune system as a means of discriminating between self and nonself.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RIG-I Antibody (NBP2-27109)(2)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 1 application: WB.


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Video Protocols

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Bioinformatics

Gene Symbol DDX58
Entrez