Western Blot: RIG-I Antibody (2M6F10) [NBP2-27109] - Analysis of RIG-I in Jurkat cell lysate in the A) absence and B) presence of immunizing peptide and C) HEK 293 cell lysate using NBP2-27109 at 10 ug/ml.
Immunohistochemistry: RIG-I Antibody (2M6F10) [NBP2-27109] - Analysis of RIG-I in formalin-fixed, paraffin-embedded human kidney using this antibody at 5 ug/ml.
Simple Western: RIG-I Antibody (2M6F10) [NBP2-27109] - Simple Western lane view shows a specific band for RIG-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Porcine reactivity reported in scientific literature (PMID: 27463126). The amino acid sequence used as immunogen is 100% homologous in human, chimp, orangutan and rhesus monkey, 85% homologous in pig, 79% homologous in mouse and horse and 73% homologous in rat.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for RIG-I Antibody (2M6F10)
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DEAD box protein 58
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
probable ATP-dependent RNA helicase DDX58
retinoic acid inducible gene I
Retinoic acid-inducible gene 1 protein
Retinoic acid-inducible gene I protein
RNA helicase RIG-I
RIG-I, an RNA helicase of the DEAD/H box family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure and in innate immune defense against viruses. It acts as a specific sensor for dsRNA, which is absent in uninfected cells but known to be accumulated in virus-infected cells. It contains two CARD domains, one helicase ATP-binding domain and the other helicase C-terminal domain. The CARD domains actually inhibit the activity of RIG-I in normal cell, but once the cell gets infected, RIG I directly binds uncapped 5-prime-triphosphate RNA present in viruses triggering an immune response signal. The signaling cascade involves MAVS/IPS1 which induces the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5) during viral replication through activation of NF-kappa-B, IRF3 and IRF7. The ability of RIG I to sense 5-prime-phosphorylated RNA evolved in the innate immune system as a means of discriminating between self and nonself.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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