Ribonuclease Inhibitor Antibody (1H23) - BSA Free

Western Blot: Ribonuclease Inhibitor Antibody (1H23) [NBP1-30167] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RNH1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (1H23) [NBP1-30167] - Analysis of RNH1 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RNH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1H23
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Ribonuclease Inhibitor Antibody (1H23) - BSA Free Summary

• Description: Novus Biologicals Mouse Ribonuclease Inhibitor Antibody (1H23) - BSA Free (NBP1-30167) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant Ribonuclease inhibitor (7-461aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNH1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

• Placental ribonuclease inhibitor
• Placental RNase inhibitor
• PRI
• RAIMGC4569
• ribonuclease inhibitor
• ribonuclease/angiogenin inhibitor 1MGC18200
• ribonuclease/angiogenin inhibitor
• RNHMGC54054

Background

Ribonuclease inhibitor 1(RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNH1
• Entrez:
  • 6050(Human)
• Uniprot:
  • NP_002930(Human)