RhoGAP Antibody (OTI9H2) [mFluor Violet 500 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI9H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 500 SE

RhoGAP Antibody (OTI9H2) [mFluor Violet 500 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 948-1261 of human ARHGAP5 (NP_001164) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGAP5
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-44958. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: >95%

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RhoGAP Antibody (OTI9H2) [mFluor Violet 500 SE]

• growth factor independent 2
• p100 RasGAP-associated p105 protein
• p105 RhoGAP
• p190-BGFI2
• p190BRhoGAP
• Rho GTPase activating protein 5
• rho GTPase-activating protein 5
• RHOGAP5
• Rho-type GTPase-activating protein 5

Background

The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGAP5