RhoGAP Antibody (OTI9H2)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI9H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RhoGAP Antibody (OTI9H2) Summary

• Description: Novus Biologicals Mouse RhoGAP Antibody (OTI9H2) (NBP3-44757) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 948-1261 of human ARHGAP5 (NP_001164) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RhoGAP
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-2000

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Lyophilized powder ( 1X PBS, pH 7.3,)
• Preservative: 8% Trehalose
• Purity: >95%
• Reconstitution Instructions: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RhoGAP Antibody (OTI9H2)

• growth factor independent 2
• p100 RasGAP-associated p105 protein
• p105 RhoGAP
• p190-BGFI2
• p190BRhoGAP
• Rho GTPase activating protein 5
• rho GTPase-activating protein 5
• RHOGAP5
• Rho-type GTPase-activating protein 5

Background

The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RhoGAP