RhoA Antibody (7E7H4) - BSA Free

Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.

Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.

Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Jurkat cells using RhoA mouse mAb (green) and negative control (red).

Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against HEK293 (1) and RhoA (AA: 1-190)-hIgGFc transfected HEK293-6e (2) cell lysate.

ELISA: RhoA Antibody (7E7H4) [NBP3-27177] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Immunocytochemistry/Immunofluorescence: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded gastric cancer tissues using RhoA mouse mAb with DAB staining.

Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded endometrial carcinoma tissues using RhoA mouse mAb with DAB staining.

Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of K562 cells using RhoA mouse mAb (green) and negative control (red).

Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of HepG2 cells using RhoA mouse mAb (green) and negative control (red).

Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 7E7H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RhoA Antibody (7E7H4) - BSA Free Summary

• Description: Novus Biologicals Mouse RhoA Antibody (7E7H4) - BSA Free (NBP3-27177) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human RhoA (AA: 1-190) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RhoA Antibody (7E7H4) - BSA Free

• h12
• oncogene RHO H12
• ras homolog gene family, member A
• Rho cDNA clone 12
• RHO12
• RhoA
• RHOH12ARH12ARHAAplysia ras-related homolog 12
• small GTP binding protein RhoA
• transforming protein RhoA

Background

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOA