RHBDD1 Antibody - BSA Free

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] - WB Validation in Mouse Heart.Loading: 15 ug of lysate Antibodies: RHBDD1, 1 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 5 min

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] - WB Validation in A549 Cells.Loading: 15 ug of cell lysate Antibodies: RHBDD1, 1 ug/ml, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RHBDD1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RHBDD1 Antibody - BSA Free (NBP1-76248) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1. The immunogen is located within the last 50 amino acids of RHBDD1. Amino Acid Squence: WDRGNTRNSPPPYG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHBDD1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
• Control Peptide:

  RHBDD1 Peptide (NBP1-76248PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RHBDD1 Antibody - BSA Free

• DKFZp547E052
• EC 3.4.21
• EC 3.4.21.-
• MGC117258
• rhomboid domain containing 1
• rhomboid domain-containing protein 1

Background

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHBDD1
• Uniprot:
  • AAI01265(Human)