Renin R Overexpression Lysate (Native)

Images

 
Western Blot: Renin receptor Overexpression Lysate (Adult Normal) [NBL1-07832] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for Renin receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Renin R Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210275 and based on accession number NM_005765.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens ATPase, H+ transporting, lysosomal accessory protein 2 (ATP6AP2), mRNA.
Gene
ATP6AP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Renin R Overexpression Lysate (Native)

  • APT6M8-9
  • ATP6AP2
  • ATP6IP2
  • ATP6M8-9
  • ATP6M8-9MRXE
  • ATPase H(+)-transporting lysosomal accessory protein 2
  • ATPase H(+)-transporting lysosomal-interacting protein 2
  • ATPase, H+ transporting, lysosomal accessory protein 2
  • ATPase, H+ transporting, lysosomal interacting protein 2
  • CAPER
  • ELDF10
  • Embryonic liver differentiation factor 10
  • ER-localized type I transmembrane adaptor
  • H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9
  • HT028
  • M8-9
  • MGC99577
  • MRXE
  • MSTP009
  • N14F
  • Renin R
  • renin receptor
  • ReninR
  • vacuolar proton ATP synthase membrane sector associated protein M8-9
  • V-ATPase M8.9 subunit
  • XMRE

Background

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Renin R Lysate (NBL1-07832) (0)

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Bioinformatics

Gene Symbol ATP6AP2