Renin R Antibody

Western blot shows lysates of human and mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renin R at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Renin R Antibody Summary

• Immunogen: E. coli-derived recombinant human Renin R
  Asn17-Glu302
  Accession # O75787
• Specificity: Detects human and mouse Renin R in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATP6AP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Renin R Antibody

• APT6M8-9
• ATP6AP2
• ATP6IP2
• ATP6M8-9
• ATP6M8-9MRXE
• ATPase H(+)-transporting lysosomal accessory protein 2
• ATPase H(+)-transporting lysosomal-interacting protein 2
• ATPase, H+ transporting, lysosomal accessory protein 2
• ATPase, H+ transporting, lysosomal interacting protein 2
• CAPER
• ELDF10
• Embryonic liver differentiation factor 10
• ER-localized type I transmembrane adaptor
• H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9
• HT028
• M8-9
• MGC99577
• MRXE
• MSTP009
• N14F
• Renin R
• renin receptor
• ReninR
• vacuolar proton ATP synthase membrane sector associated protein M8-9
• V-ATPase M8.9 subunit
• XMRE

Background

Renin R (Renin Receptor; also ATPase H⁺-transporting lysosomal accessory/interacting protein 2, M8.9 and ELDF10) is a 39‑45 kDa protein that belongs to no known family. It is expressed on the surface of macrophages, vascular smooth muscle cells, renal mesangial cells and T cells. Renin R has at least two functions. First, it binds both renin and prorenin, promoting the conversion of angiotensinogen to angiotensin I. Second, its ligation induces PAI‑1 synthesis. Mature human Renin R is a type I transmembrane protein 334 amino acids (aa) in length. It contains a 286 aa extracellular region (aa 17‑302) and a 27 aa cytoplasmic tail. It is believed to form homodimers. Potential isoforms exist. One termed M8.9 shows a deletion of aa 1‑252 and constitutes a component of the vacuolar proton ATPase. Two others show an alternate start site at Met77, and a deletion of aa 101‑132. Over aa 17‑302, the human Renin R shares 95% aa identity with the mouse Renin R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renin R Antibody (AF5716)(5)

Publications using AF5716

• Lachlan G. Schofield, Richard G. S. Kahl, Samantha L. Rodrigues, Joshua J. Fisher, Saije K. Endacott, Sarah J. Delforce et al. Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity Frontiers in Cell and Developmental Biology 2023-08-01 [PMID: 37588662]
• Lachlan G. Schofield, Richard G. S. Kahl, Samantha L. Rodrigues, Joshua J. Fisher, Saije K. Endacott, Sarah J. Delforce et al. Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity Frontiers in Cell and Developmental Biology 2023-08-01 [PMID: 37588662] (Immunohistochemistry, Western Blot, Mouse)
• Figueiredo M, Daryadel A, Sihn G Et Al. The (pro)renin receptor (ATP6ap2) facilitates receptor-mediated endocytosis and lysosomal function in the renal proximal tubule Pflugers Archiv : European journal of physiology 2021-08-01 [PMID: 34228176]
• Shinji Yamashita, Kazal Boron Biswas, A H M Nurun Nabi, Tsutomu Nakagawa, Fumiaki Suzuki, Akio Ebihara Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells Biomedical Reports 2018-07-05 [PMID: 30271601]
• Zhen Li, Lili Zhou, Yongping Wang, Jinhua Miao, Xue Hong, Fan Fan Hou et al. (Pro)renin Receptor Is an Amplifier of Wnt/ beta -Catenin Signaling in Kidney Injury and Fibrosis Journal of the American Society of Nephrology 2017-08-01 [PMID: 28270411]

Bioinformatics

• Gene Symbol: ATP6AP2
• Uniprot:
  • O75787()