RelA/NFkB p65 NLS Antibody - BSA Free

Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody [NBP2-24541] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using p65 NLS antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Bv, Eq, Pm, Rt, Ca, Ch, Pm, Dr, Pm, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RelA/NFkB p65 NLS Antibody - BSA Free Summary

• Immunogen: A portion of the NFkB p65 NLS nuclear localization signal (NLS) (amino acids DTDDRHRIEEKRKRKT) was used as the immunogen for this antibody.
• Predicted Species: Rat (100%), Chicken (100%), Canine (100%), Xenopus (100%), Monkey (100%), Drosophila (100%), Chimpanzee (100%), Zebrafish (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml
• Control Peptide:

  RelA/NFkB p65 NLS Protein (NBP2-24541PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RelA/NFkB p65 NLS Antibody - BSA Free

• MGC131774
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65 NLS
• p65 Nuclear Localization Sequence
• p65-NLS
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RelA/NFkB p65 NLS Antibody (NBP2-24541)(5)

Publications using NBP2-24541

• Marwarha G, Slagsvold KH, Høydal MA NF-?B Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression International journal of molecular sciences 2023-04-01 [PMID: 37047592] (ELISA)
• Ren J, Hu Z, Li Q et al. Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-?B-IRF1 signaling BMC biology 2023-03-08 [PMID: 36882743] (Western Blot, Mouse)
• Song H, Ye X, Liao Y et al. NF-?B represses retinoic acid receptor-mediated GPRC5A transactivation in lung epithelial cells to promote neoplasia JCI Insight 2023-01-10 [PMID: 36413416] (Western Blot, Mouse)
• Milanovic M. Regulation of conformation and activity of nuclear NF-kappaBeta p65 by phosphorylation, chaperones and p65 DNA-binding. Thesis. 2014-03-03 (IP, Human)
  
  Details:
  Nuclear extracts of HEk-393 cells stimulated with TNF, Fig 3.8. The results showed that the p65 mAb antibody immunoprecipitated TNF-activated nuclear p65. This mAb recognizes the NLS of p65 when IkBa is degraded.
• Khan HU, Aamir K, Jusuf PR et al Lauric acid ameliorates lipopolysaccharide (LPS)-induced liver inflammation by mediating TLR4/MyD88 pathway in Sprague Dawley (SD) rats Life Sci 2020-11-14 [PMID: 33188836] (WB, Mouse)
  
  Details:
  Citation using the Azide Free version of this antibody.

Bioinformatics

• Gene Symbol: RELA