Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Suitable Sample Type: Human, mouse, and rat cell lysates
• Standard Curve Range: N/A
• Sensitivity: N/A

Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) Summary

• Description: The NF?B p65 ELISA kit allows the accurate measurement of total and phosphorylated human NFkB p65 in cell lysates. This ELISA uses the traditional sandwich ELISA format, but with a major difference: both the sample and the assay reagents can be added to the assay microplate at the same time. Since the antibodies are not precoated in the wells, several different targets can be analyzed simultaneously in the same plate in different wells. With this kit a mixture of capture and detection antibodies against either phosphorylated or total NFkB can be added to the sample lysate, thus enabling their detection with a simple 1-hour protocol.
• Standard Curve Range: N/A
• Sensitivity: N/A
• Assay Type: Sandwich ELISA
• Inter-Assay: N/A
• Intra-Assay: N/A
• Spike Recovery: N/A
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: RELA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

Stimulation of the canonical NFkB pathway leads to the phosphorylation of and subsequent degradation of IkB; phosphorylation of the latter is controlled by a specific set of proteins referred to as the I Kappa Kinases (IKK), which include IKKa and IKKb. Under normal conditions, IkB acts as a key regulatory molecule in that it sequesters p65 or Rel A within the cytoplasm; this blocks NFkB dependent transcription. Upon phosphorylation of IkB by IKKs, p65 is released and free to associate with the p50 subunit; p65 acts as a transactivator whereas p50 possesses DNA binding activity. Complex formation leads to the subsequent translocation of the NFkB complex into the nucleus and the concomitant up-regulation of NFkB dependent genes. Disruption of this signaling nexus has been documented in a number of human pathological conditions including inflammation, infection and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for RelA/NFkB p65 ELISA Kit (NBP2-62162). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: RELA