Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Suitable Sample Type: Human, mouse, and rat cell lysates
• Standard Curve Range: N/A
• Sensitivity: N/A

Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) Summary

• Description: The NF?B p65 ELISA kit allows the accurate measurement of total and phosphorylated human NFkB p65 in cell lysates. This ELISA uses the traditional sandwich ELISA format, but with a major difference: both the sample and the assay reagents can be added to the assay microplate at the same time. Since the antibodies are not precoated in the wells, several different targets can be analyzed simultaneously in the same plate in different wells. With this kit a mixture of capture and detection antibodies against either phosphorylated or total NFkB can be added to the sample lysate, thus enabling their detection with a simple 1-hour protocol.
• Standard Curve Range: N/A
• Sensitivity: N/A
• Assay Type: Sandwich ELISA
• Inter-Assay: N/A
• Intra-Assay: N/A
• Spike Recovery: N/A
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: RELA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

Stimulation of the canonical NFkB pathway leads to the phosphorylation of and subsequent degradation of IkB; phosphorylation of the latter is controlled by a specific set of proteins referred to as the I Kappa Kinases (IKK), which include IKKa and IKKb. Under normal conditions, IkB acts as a key regulatory molecule in that it sequesters p65 or Rel A within the cytoplasm; this blocks NFkB dependent transcription. Upon phosphorylation of IkB by IKKs, p65 is released and free to associate with the p50 subunit; p65 acts as a transactivator whereas p50 possesses DNA binding activity. Complex formation leads to the subsequent translocation of the NFkB complex into the nucleus and the concomitant up-regulation of NFkB dependent genes. Disruption of this signaling nexus has been documented in a number of human pathological conditions including inflammation, infection and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for RelA/NFkB p65 ELISA Kit (NBP2-62162). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: RELA